Journal: BMC Genomics

Article Title: A versatile 5′ RACE-Seq methodology for the accurate identification of the 5′ termini of mRNAs

doi: 10.1186/s12864-022-08386-y

Figure Lengend Snippet: Comparative analysis of aligned sequencing reads on the human BCL2L12 gene, derived from the presented 5′ RACE-seq approach and publicly available Direct RNA sequencing datasets. A Visualization of the aligned sequencing reads derived from the presented 5′ RACE-seq approach. B Visualization of the aligned sequencing reads derived from publicly available Direct RNA sequencing datasets deposited on the Sequence Read Archive database (SRA). C Indicative raw nanopore sequencing reads from the generated FASTQ file of the presented 5′ RACE-seq approach, highlighting the incorporation of the drTSO specifically to the 5′ end of each target mRNA transcript

Article Snippet: Our results led to the accurate identification of the 5′ UTRs of the BCL2L12 gene, being in accordance with the 5′ ends provided by both direct RNA and PCR-cDNA sequencing method from Oxford Nanopore Technologies.

Techniques: Sequencing, Derivative Assay, RNA Sequencing, Nanopore Sequencing, Generated

Journal: BMC Genomics

Article Title: A versatile 5′ RACE-Seq methodology for the accurate identification of the 5′ termini of mRNAs

doi: 10.1186/s12864-022-08386-y

Figure Lengend Snippet: Template-switching oligonucleotides (TSOs) and 5′ RACE primers used in the present work

Article Snippet: Our results led to the accurate identification of the 5′ UTRs of the BCL2L12 gene, being in accordance with the 5′ ends provided by both direct RNA and PCR-cDNA sequencing method from Oxford Nanopore Technologies.

Techniques: Sequencing, Modification